Protein-DNA cross-linking at thelacpromoter
نویسندگان
چکیده
منابع مشابه
Protein-DNA cross-linking at the lac promoter.
We report the results of photo-cross-linking of RNA polymerase and the cyclic AMP receptor protein (CRP) to the lac UV5 promoter region carried on either a linear fragment or a supercoiled plasmid. We have devised a protocol that allows the localisation of bases in contact with the protein. RNA polymerase makes contacts within the -10 and -35 regions of the promoter, essentially on the non-temp...
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The induction of DNA cross-linking in mammalian cells by various carcinogens was investigated by the method of alkaline elution. A dose-dependent increase in DNA cross-linking was seen following exposure of human fibroblasts to N-acetyoxy-2-acetylaminofluorene and following exposure of mouse embryo cells to 7,12-dimethylbenz[a]-anthracene. No cross-link effect was seen following treatment with ...
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1,3-Propanesultone produces in vitro complexes between DNA and DNA, DNA and RNA, RNA and RNA, DNA and proteins, and possibly RNA and proteins. All these interactions were detected with a variety of independent analytical techniques. Increased attachment of the 1,3-pro panesultone-treated DNA to the microsomal membrane and enhanced adsorption to and penetration into Ehrlich ascites tumor cells w...
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Interaction of the Tramtrack protein from Drosophila melanogaster with DNA was analyzed by a cross-linking method. Tramtrack residues cross-linkable to the partially depurinated DNA were identified by direct sequencing. The N-terminal alpha-amino group of the protein DNA-binding domain was found to be the major product of cross-linking. The location of the N terminus on the DNA was determined b...
متن کاملDNA-Protein Cross-Linking by Chemical Carcinogens in Mammalian Cells1
The induction of DNA cross-linking in mammalian cells by various carcinogens was investigated by the method of alkaline elution. A dose-dependent increase in DNA cross linking was seen following exposure of human fibroblasts to N-acetoxy-2-acetylaminofluorene and following expo sure of mouse embryo cells to 7,12-dimethylbenz[a]anthracene. No cross-link effect was seen following treat ment with ...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1991
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/19.4.833